Coding
Part:BBa_K2476010:Design
Designed by: Vivian Hu Group: iGEM17_Pittsburgh (2017-10-27)
pdDronpa1.2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for Escherichia coli K12 and checked for BioBrick restriction sites using IDT Codon Optimization Tool.
pdDronpa1.2 is a photodissociable dimer designed by the Lin Laboratory at Stanford University that is switched off by 500-nm cyan light and on by 400-nm violet light. This engineered Dronpa is able to monomerize under 500-nm light and dimerize under 400-nm lab. The Lin Laboratory has been able to control kinase activity by flanking a kinase active site with two monomeric Dronpa.
Source
pdDronpa1.2 as designed and used by the Lin Laboratory in http://science.sciencemag.org/content/355/6327/836.full. Synthesized by IDT.