Coding

Part:BBa_K2476010:Design

Designed by: Vivian Hu   Group: iGEM17_Pittsburgh   (2017-10-27)


pdDronpa1.2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Escherichia coli K12 and checked for BioBrick restriction sites using IDT Codon Optimization Tool.

pdDronpa1.2 is a photodissociable dimer designed by the Lin Laboratory at Stanford University that is switched off by 500-nm cyan light and on by 400-nm violet light. This engineered Dronpa is able to monomerize under 500-nm light and dimerize under 400-nm lab. The Lin Laboratory has been able to control kinase activity by flanking a kinase active site with two monomeric Dronpa.

Source

pdDronpa1.2 as designed and used by the Lin Laboratory in http://science.sciencemag.org/content/355/6327/836.full. Synthesized by IDT.

References